Gene expression profiling of HepG2 cells after treatment with black tea polyphenols

Black tea polyphenols on gene expression profiling

  • shuangxiang tan Hunan Provincial People’s Hospital
Keywords: Black tea polyphenols; Microarray; HepG2 cells; Gene expression

Abstract

Purpose: To determine the effects of black tea polyphenols on gene expression in hepatocellular cancer cells.

Methods: The total RNA from HepG2 hepatocellular cancer cells untreated and treated with black tea polyphenols was subjected to Human 14K cDNA microarray analysis. Real-time PCR and Western blot analysis were conducted to verify microarray data.

Results: A total of 48 genes showed more than two-fold change after black tea polphenols treatment, and they were involved in the regulation of cell growth, cell cycle, apoptosis, signaling, angiogenesis, tumor invasion and metastasis. Real-time PCR analysis of the selected genes showed that their mRNA expression changes were consistent with the microarray data. In addition, Western blot analysis of the selected genes showed that their protein expression changes were consistent with mRNA expression.

Conclusion: The gene expression profiles provide comprehensive molecular mechanisms by which black tea polyphenols exerts growth inhibition effects on cancer cells. The novel molecular targets identified in this study may be further exploited as therapeutic strategies for hepatocellular cancer.

Published
2019-08-07
Section
Articles