Experimental Characterization of MCF-10A Normal Cells Using AFM: Comparison with MCF-7 Cancer Cells
The mechanical properties of single cells have been recently identified as the basis of an emerging approach in medical applications because they are closely related to the biological processes of cells and, ultimately, human health conditions. The problem is how to measure mechanical properties to be more accurate and applicable. Some of cell’s properties such as elasticity module and adhesion have been measured before using various methods but comprehensive tests for two healthy and cancerous cells have not been done simultaneously. On the other hand, AFM as a Nanoscale device has been used for some other biological cells but for breast cells it has been just used to measure elasticity module. To have more accurate comparison for healthy and malignant cancer cells of the breast, mechanical properties of MCF-7 and MCF-10A cells such as topography, elasticity module, adhesion force, viscoelastic characteristics, bending rigidity and axial rigidity were determined. AFM as an accurate device used to accomplish tests. Results showed that healthy breast cells are stiffer and less adhesive in comparison with cancerous ones. Moreover, topography images revealed that cancerous cells have bigger radiuses and geometrically are more regular. These results can help diagnosis of malignant cancer cells and even the level of the disease.
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