Total phenols, flavonoids and anti-radical activity in Annona muricata and Annona purpurea callus culture

  • CARLOS ALBERTO LECONA GUZMAN INSTITUTO TECNOLOGICO DE TUXTLA GUTIERREZ
  • Marient Yareli Ovando Dominguez INSTITUTO TECNOLÓGICO DE TUXTLA GUTIÉRREZ
  • María Celina Lujan Hidalgo, MC INSTITUTO TECNOLÓGICO DE TUXTLA GUTIÉRREZ
  • Daniel González Mendoza, DR
  • Arely Anayansi Vargas Díaz, DRA CONACYT−Colegio de Postgraduados, Campus Campeche
  • Nancy Ruiz Lau, DRA
  • Federico Antonio Gutiérrez Miceli, DR
Keywords: Annonaceae, Antioxidant Activity, 6-benzyladenine, Secondary Metabolites, Tissue Culture

Abstract

Callus cultures of Annona muricata and Annona purpurea were induced in Murashige and Skoog (MS) medium supplemented with different concentrations of 1-naphthylacetic acid (NAA), 6-benzyladenine (BA) and 2,4-dichlorophenoxyacetic acid (2,4-D) utilized hypocotyls with explant. The highest percentage of callus formation frecuency was the treatment supplemented with 3 mgL-1 NAA for A. muricata (100%) while for A. purpurea in lower percentage (75%). BA stimulated the formation of shoots in all the evaluated concentrations, being the concentration of 2 mgL-1 the one that induced the greater formation of shoots for A. muricata (23 shoots/explant) and A. purpurea (28 shoots/explant). The content of total phenols, flavonoids and antiradical activity was measured in the callus obtained from both species. The results showed that the callus of A. purpurea have a higher content of total phenols (27.8 mgg-1 dw) compared to A. muricata (23.2 mgg1 dw). The highest content of total flavonoids was observed in the callus of A. purpurea (8.0 μgg-1 dw). Anti-radical was determined by the 2,2-Diphenyl-1-Picrylhydracil Radical assay. The concentration required for 50% inhibition (IC50) of the 2,2-Diphenyl-1-Picrylhydracil radicals were 4.22 μgmL-1 in methanolic extracts of callus of A. muricata, while in extracts of callus of A. purpurea was 2.86 μgmL-1, in both cases was greater than that found for leaves. Callus culture of the species studied in this work represents an alternative for the production of natural antioxidants.

Published
2019-08-07
Section
Articles