The Effects of melatonin on growth, physiology and gene expression in rice seedlings under Cadmium stress

  • Xiachen Lv
  • Yunxia Fang
  • Lantian Zhang
  • Weiyi Zhang
  • Ling Xu
  • Jingjin Han
  • Xian Zhang
  • Xiaoqin Zhang
  • Dawei Xue Hangzhou Normal University
Keywords: Rice; Melatonin; Cadmium stress; Physiological and biochemical index; Gene expression

Abstract

Melatonin (MLT) is a hormonal substance found in many organisms and can improve plant stress resistance. In this study, the japonica rice variety Y32 and indica rice variety NJ6 were cultivated in hydroponics under different concentrations of CdCl2 at the two-leaf stage. The growth, physiological and biochemical responses of the seedlings and the expression of cadmium (Cd)-related genes under exogenous melatonin (MLT) treatment were assessed. The results indicated that Cd stress destroyed the dynamic balance between reactive oxygen species (ROS) production and removal, resulting in ROS accumulation, membrane lipid peroxidation, and impaired growth and development. Following the application of exogenous MLT to rice seedlings, increases in plant biomass including both underground and above-ground areas were observed. MLT also relived the inhibition of superoxide dismutase (SOD) and peroxidase (POD) in a concentration dependent manner in response to Cd stress. Catalase (CAT) activity and malondialdehyde (MDA) expression also decreased following MLT treatment. Amongst the six Cd-related genes assessed, five genes were down-regulated and one was up-regulated in response to MLT treatment. Taken together, these data demonstrate that MLT improves the resilience of rice seedlings at the biochemical, physiological, and molecular levels, and alleviates the damage caused by Cd stress.

Published
2019-08-07
Section
Articles