Genome-Wide Identification and Expression Profiling of Brassica napus Invertase Genes Suggest Roles in Silique Development and the Response to Sclerotinia sclerotiorum

  • Jingsen Liu ollege of Agronomy and Biotechnology, Southwest University
  • Jinqi Ma
  • Ai Lin
  • Chao Zhang
  • Bo Yang
  • Liyuan Zhang
  • Ling Huang
  • Jiana Li

Abstract

 Invertase (INV) is one of the key enzymes in plant sucrose metabolism. It irreversibly catalyzes the hydrolysis of sucrose to glucose and fructose, and thus plays an important role in plant growth, development, and in responses to biotic and abiotic stress. In this study, we identified 27 members of the BnINV family in Brassica napus. We constructed a phylogenetic tree of the family and predicted the gene structures, conserved motifs, promoter cis-acting elements, physicochemical protein properties, and chromosomal distributions of the BnINVs. We also analyzed the expression of the BnINVs in different tissues and developmental stages in the B. napus cultivar Zhongshuang 11 using qRT-PCR. In addition, we analyzed RNA-sequencing data to explore the expression patterns of the BnINVs in four different harvest index cultivars and inoculated with the pathogenic fungus Sclerotinia sclerotiorum. We used a weighted coexpression network analysis to explore the BnINV regulatory networks. Finally, we selected some BnINV genes and explored their expression patterns in long (Zhongshuang 4) and short (Ningyou 12) silique rapeseed cultivars. Our results showed that the BnINVs might play important roles in the growth and development of rapeseed siliques and the defense response against pathogens.

Published
2020-05-21
Section
Articles